Gold nanoparticles and electromagnetic irradiation in tissue culture systems of bleeding heart: biochemical, physiological, and (cyto)genetic effects

Abstract This study aimed to analyze the effect of various mutagens on in vitro development, physiological activity, acclimatization efficiency, and genetic integrity Lamprocapnos spectabilis ‘Valentine’. Gold nanoparticles (AuNPs), microwaves, X-rays were used at different doses. The profiles primary secondary metabolites enzymatic activity produced plants studied. usefulness markers detection mutations species was compared. genome size L. estimated for first time. It found that addition AuNPs into culture medium had a positive impact development multiplication plants. All shoots regenerated adventitious roots, but subjected longest microwave irradiation (3 × 9 s) non-treated control lowest efficiency. Application significantly affected profile most enzymes phytochemicals studied, however, final depended agent type dose. Mutations detected by DAMD, RAPD, SCoT 7.5% plants, not ISSRs. Phenotype variation leaf shape four be very small; about 1281 Mbp.